Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p209 | (1) | WCRB2014

Effect of local treatment of seminal vesiculitis on the quality of equine frozen semen

Sancler-Silva Yame F R , Monteiro Gabriel A , Neto Carlos Ramires , Freitas Dell'aqua Camila P , Crespilho Andre M , Papa Frederico O , Oliveira Pedro V

The seminal vesiculitis leads to contamination of the semen with inflammatory cells, causing agglutination, precipitation, decreased sperm motility and increased reactive oxygen species (ROS). This deleterious effect is most pronounced during the cryopreservation process. Therefore, the aim of this study was to evaluate the quality of frozen semen from stallions with seminal vesiculitis after local treatment. Five stallions with seminal vesiculitis were used and local treatmen...

ra0001p248 | (1) | WCRB2014

Comparision of DNA fragmentantion and plasma membrane integrity between chilled and frozen semen of bulls

de Mello Papa Patricia , Neto Carlos Ramires , Guasti Priscilla Nascimento , Maziero Rosiara Rosaria Dias , Sancler-Silva Yame F R , Monteiro Gabriel A , Dell'aqua Camila P Freitas , Junqueira Cassio Renesto , Martin Ian , Alvarenga Marco Antonio , Papa Frederico O , Aqua Junior Jose Antonio Dell'

In fixed time artificial insemination (FTAI) is frozen semen is commonly used, however the use of cooled semen is a practice alternative to reduce costs. The aim of this study was compare the difference in DNA fragmentation and plasma membrane integrity between chilled and frozen semen of bulls. Two ejaculates from 20 bulls were collected. Semen was diluted in extender with egg-yolk and glycerol (Botu-Bov™) and divided in two groups: chilled group (CG) samples were cooled...